A brief comparative bioinformatics analysis of a KatB-like catalase protein candidate from Bacillus thuringiensis Bt26 and reference KatB proteins from other B. thuringiensis strains

Aliyev Zafar Zokirovich; Khalilov Ilkhom Mamatkulovich; Bobokulov M.Sh.; Abdullayev A.K.

doi:10.66073/ReFocus-Conf/3010034

ARTICLE 2026 Vol 1 · No 8 EN eefe4b129ffc

A brief comparative bioinformatics analysis of a KatB-like catalase protein candidate from Bacillus thuringiensis Bt26 and reference KatB proteins from other B. thuringiensis strains

Краткий сравнительный биоинформатический анализ белка-кандидата KatB-подобной каталазы Bacillus thuringiensis Bt26 и референсных белков KatB других штаммов B. thuringiensis | Bacillus thuringiensis Bt26 shtammining KatB-ga o‘xshash katalaza oqsili nomzodi va boshqa B. thuringiensis shtammlarining referens KatB oqsillarining qisqacha qiyosiy bioinformatik tahlili

Aliyev Zafar Zokirovich Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan
Khalilov Ilkhom Mamatkulovich Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan
Bobokulov M.Sh. Rice Research Institute
Abdullayev A.K. Rice Research Institute

Partner conferences of the International Scientific Journal Research Focus, 1(8), 127–132

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Abstract

This study comparatively analyzed the catalase protein candidate corresponding to the JEEBFNLG_01767 locus in the genome of Bacillus thuringiensis 26 against reference KatB protein sequences from 50 other B. thuringiensis strains. BLASTP analysis revealed amino acid sequence identity ranging from 94.672% to 100%, with 27 reference proteins showing 100% identity. BLASTN analysis identified 26 reference sequences with 100% nucleotide identity. Multiple sequence alignment revealed 40 variable amino acid positions and a total of 125 recorded differences, with the most frequent differences occurring at positions 254, 169, and 291. Phylogenetic analysis indicated close sequence relationships between the protein candidate and many reference proteins. However, despite being annotated as katA_1, the locus exhibited high similarity to reference KatB proteins. Therefore, the protein is provisionally described as a KatB-like catalase candidate, and further studies are required to clarify its functional identity.

Keywords

Bacillus thuringiensis Bt26 catalase KatB protein amino acid substitutions comparative bioinformatics

How to cite

Aliyev Zafar Zokirovich; Khalilov Ilkhom Mamatkulovich; Bobokulov M.Sh.; Abdullayev A.K. (2026). A brief comparative bioinformatics analysis of a KatB-like catalase protein candidate from Bacillus thuringiensis Bt26 and reference KatB proteins from other B. thuringiensis strains. Partner conferences of the International Scientific Journal Research Focus, 1(8), 127–132. https://doi.org/10.66073/ReFocus-Conf/3010034

Identifier: https://rf-library.uz/article/eefe4b129ffc/a-brief-comparative-bioinformatics-analysis-of-a-katb-like-catalase-protein
DOI: 10.66073/ReFocus-Conf/3010034
Licence: Copyright (c) 2026 Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan; Authors
Publisher: LLC Ilm-fan va innovatsiyalar akademiyasi
Added to repository: 2026-11-03