Bacillus thuringiensis Bt1Fo shtammining tanlangan cry1Ia geni uchun mutagen praymerlarni loyihalash va PCR tahlili

Reyimberdiyeva Marjon Zafar qizi; Karimov Nurbek Rayimovich; Rahimova Sh.Sh.; Kobilov Fazliddin Bozorovich

doi:10.66073/ReFocus-Conf/3010039

ARTICLE 2026 Vol 1 · No 8 UZ 3fddc0dba38e

Bacillus thuringiensis Bt1Fo shtammining tanlangan cry1Ia geni uchun mutagen praymerlarni loyihalash va PCR tahlili

Design of mutagenic primers for the selected cry1Ia gene of Bacillus thuringiensis strain Bt1Fo and PCR analysis | Разработка мутагенных праймеров для отобранного гена cry1Ia штамма Bacillus thuringiensis Bt1Fo и ПЦР-анализ

Reyimberdiyeva Marjon Zafar qizi Toshkent kimyo-texnologiya instituti
Karimov Nurbek Rayimovich Mirzo Ulug‘bek nomidagi O‘zbekiston Milliy universiteti
Rahimova Sh.Sh. Toshkent kimyo-texnologiya instituti
Kobilov Fazliddin Bozorovich O‘zbekiston Respublikasi Fanlar akademiyasi Mikrobiologiya instituti

Partner conferences of the International Scientific Journal Research Focus, 1(8), 155–159

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Annotatsiya

Bacillus thuringiensis bakteriyalari tomonidan ishlab chiqariladigan Cry oilasiga mansub insektitsid oqsillar qishloq xo‘jaligi zararkunandalariga qarshi biologik kurashda muhim ahamiyatga ega. Avvalgi tadqiqotlarda Cry1Ia oqsilining ayrim variantlari shakarqamishning yirik poya zararkunandasi Telchin licusga qarshi faollik ko‘rsatgan. Mazkur ishda Bt1Fo shtammining GenBank bazasida PP897829 kirish raqami bilan saqlangan cry1Ia geni asosida adabiyotlarda tavsiflangan aminokislota almashinuvlariga mos praymerlarni loyihalash va PCR natijalarini dastlabki baholash maqsad qilindi. Tadqiqot doirasida D233N, I116T, L266F va E639G mutatsiyalariga mos praymerlar tanlandi hamda PCR amplifikatsiyasi amalga oshirildi. Gel elektroforezi tasvirida to‘rtta namunaning har birida asosiy DNK tasmalari kuzatildi. Olingan natijalar PCR mahsulotlari mavjudligini dastlabki baholash imkonini berdi.

Kalit so‘zlar

Bacillus thuringiensis Cry1Ia Bt1Fo praymer dizayni PCR mutagenez

Iqtibos keltirish

Reyimberdiyeva Marjon Zafar qizi; Karimov Nurbek Rayimovich; Rahimova Sh.Sh.; Kobilov Fazliddin Bozorovich (2026). Bacillus thuringiensis Bt1Fo shtammining tanlangan cry1Ia geni uchun mutagen praymerlarni loyihalash va PCR tahlili. Partner conferences of the International Scientific Journal Research Focus, 1(8), 155–159. https://doi.org/10.66073/ReFocus-Conf/3010039

Identifikator: https://rf-library.uz/article/3fddc0dba38e/design-of-mutagenic-primers-for-the-selected-cry1ia-gene-of-bacillus
DOI: 10.66073/ReFocus-Conf/3010039
Litsenziya: Copyright (c) 2026 O‘zbekiston Respublikasi Fanlar akademiyasi Mikrobiologiya instituti; Mualliflar
Nashriyot: LLC Ilm-fan va innovatsiyalar akademiyasi
Repozitoriyga qo‘shildi: 2026-11-03