ITS-based identification and phylogenetic characterization of endophytic Alternaria sp. H8 isolated from Helichrysum mussae

Ubaydullaev Ziyokhoja; Khujanov Alisher; Fayziev Vokhid Bakhramovich; Madaminov Sulaymon; Eshboev Farhod Bakir ugli

doi:10.66073/ReFocus-Conf/3010053

ARTICLE 2026 Vol 1 · No 8 EN 2675c724d5a2

ITS-based identification and phylogenetic characterization of endophytic Alternaria sp. H8 isolated from Helichrysum mussae

Идентификация и филогенетическая характеристика эндофитного изолята Alternaria sp. H8, выделенного из Helichrysum mussae, на основе ITS-области | Helichrysum mussae dan ajratilgan endofit Alternaria sp. H8 ning ITS asosida identifikatsiyasi va filogenetik tavsifi

Ubaydullaev Ziyokhoja Chirchik State Pedagogical University
Khujanov Alisher Samarkand State University named after Sharof Rashidov
Fayziev Vokhid Bakhramovich Chirchik State Pedagogical University
Madaminov Sulaymon Institute for Advanced Studies at New Uzbekistan University
Eshboev Farhod Bakir ugli Institute for Advanced Studies at New Uzbekistan University

Partner conferences of the International Scientific Journal Research Focus, 1(8), 198–199

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Abstract

An endophytic fungal isolate, H8, recovered from Helichrysum mussae, was investigated using internal transcribed spacer (ITS) sequence analysis to clarify its taxonomic placement. A quality-trimmed, 544-bp reverse-strand ITS read was compared with reference sequences of five Alternaria type strains. Phylogenetic analysis placed H8 within the genus Alternaria. The isolate matched the A. destruens type strain across 521 aligned positions and shared 99.4–99.8% sequence identity with four additional species. Because the ITS region showed limited variation among the reference taxa, these findings support identification at the genus level but do not establish a definitive species assignment. Additional multilocus sequencing is required for species-level resolution.

Keywords

endophytic fungi Helichrysum mussae Alternaria ITS sequencing fungal identification phylogenetic analysis

How to cite

Ubaydullaev Ziyokhoja; Khujanov Alisher; Fayziev Vokhid Bakhramovich; Madaminov Sulaymon; Eshboev Farhod Bakir ugli (2026). ITS-based identification and phylogenetic characterization of endophytic Alternaria sp. H8 isolated from Helichrysum mussae. Partner conferences of the International Scientific Journal Research Focus, 1(8), 198–199. https://doi.org/10.66073/ReFocus-Conf/3010053

Identifier: https://rf-library.uz/article/2675c724d5a2/its-based-identification-and-phylogenetic-characterization-of-endophytic
DOI: 10.66073/ReFocus-Conf/3010053
Licence: Copyright (c) 2026 Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan; Authors
Publisher: LLC Ilm-fan va innovatsiyalar akademiyasi
Added to repository: 2026-11-03